Flyion
德國Flyion GmbH www.flyion.com
德國Flyion公司根據多年的傳統膜片鉗儀器生產經驗，經過反復試驗，結合國際上一些最新的研究成果，革命性的提出了Flip-Clip翻轉膜片鉗技術。通過將單細胞懸液排列成排，逐一沖入玻璃微管，從而在管尖形成穩定的GigaSeal（吉歐電阻封接）。通過信號放大器可以測量，這種方式形成的GigaSeal與通過吸細胞得到的GigaSeal完全一樣，可以進行后續離子通道研究。這種方式形成的膜片鉗完全排除了顯微鏡和顯微操作，從而第一次實現了膜片鉗技術的全自動化。
flyion GmbH was founded by Dr. Albrecht Lepple-Wienhues and Dr. Klaus Ferlinz in 2001 as a spin-off of the University of Tübingen (Institute of Physiology). The founders were supported by i.con.innovation.
In 1991, Erwin Neher and Bert Sakmann won the Nobel Prize in Medicine for their groundbreaking study of ion channels. In its conventional form, the patch clamp process that they developed is extremely labour intensive and demands a high degree of manual dexterity in order to yield reliable results. flyion has simplified this procedure with its patented Flip-the-Tip™ technology, which is ideally suited for automation.
The company offers a range of products based on this technology:
· The Flyscreen® 8500 is a patch clamp robot that performs high-quality ion channel screenings automatically. Several compounds can be tested one after the other.
· The PatchBox™ is a cost-effective plug&play workstation tool that can be combined with existing amplifiers, pullers and software. It is simple to operate and delivers a high success rate in the automatic formation of gigaseals and whole-cell configurations in a standard patch pipette.
· The ChipTip™ recording tips for the Flyscreen® 8500 consist of a glass pipette in a plastic casing. They ensure high success rates and allow extremely fast solution exchange within the pipette while keeping volumes to an absolute minimum for the study of the fastest ligand-dependent ion channels.
· The Feedback Microforge is a fully automatic micro glass pressure polishing system for the production of special glass pipette shapes in patch clamp technology.
As a solutions provider, and to complement its product range, flyion also offers the following services that help ensure the company’s products are used efficiently:
· hERG safety analysis of active substances
· Ion channel screening to help discover new active substance candidates
· Creation and characterisation of stably transfixed, inducible cell lines
· Optimisation of assays for automated ion channel screenings
